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In Heat Shock Proteins and Whole Body Physiology, eds

: Off Label Pseudophakic patients with glaucoma (Schmidt et al 2010) A 2010 study (Schmidt et al, 2010) concluded that echothiophate may be effective in lowering IOP in patients with advanced glaucoma that have had previous cataract surgery to replace their eye's natural cloudy lens with a clear aritificial intraocular lens (pseudophakic patients), and for whom maximal medical therapy with newer class medications provides insufficient lowering of IOP

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Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS