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10.1016/j.jtemb.2018.04.005 63 VenardosK

Proliferation Assay Fresh isolated PBMCs or purified T/B cells labeled carboxyfluorescein succinimidyl ester (CFSE) were cultured with different concentration of L-Cn (Sigma-Aldrich) for 5 days in the presence of soluble anti-CD3 (5 g/mL, Biolegend) and anti-CD28 (5 g/mL, Biolegend) for T cell proliferation assay or 7 days in the presence of CPG (10 g/mL, InvivoGen) for B cell proliferation assay or with medium only as control

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17 Its structure, which is primarily GIP sequence-based, includes a C20 fatty di-acid acyl chain that extends the duration of action, thereby allowing once-weekly subcutaneous administration