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The pH titration experiments were performed in 100 mM HEPES, 150 mM NaCl 2 , 0.5 mM MgCl 2 and pH between 6 and 9 adjusted by KOH, with the actual pH measured post-hoc

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It was reported that mammalian expression systems have potential disadvantages, such as viral contamination of target proteins and bioethical concerns, which may lead some researchers to explore alternative expression systems such as yeast, filamentous fungi, and higher plants

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