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The effect on platelet COX-1 activity is permanent for the lifetime of the platelet, since platelets lack DNA and cannot synthesize new enzyme

Colorimetric/fluorometric assay kits [Biovision Incorporated, Milpitas, USA] were used to measure aspartate aminotransferase (AST), alanine aminotransferase (ALT) and -glutamyltransferase (-GGT)