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After 72 h of methanol induction, the expressed extracellular GSTA2X protein samples were separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by staining with Coomassie Brilliant Blue R-250 and silver staining reagent (Beyotime Biotechnology, Shanghai, China)
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The activation of NRF-2 in liver suppresses the synthesis of fatty acids [70], as observed in this study that included experimental feeding with 8-week-old NRF-2 null mice, and wild-type mice fed experimentally, observing the mRNA of sterol regulatory element-binding protein 1-c and fatty acid synthase as highly expressed in NRF-2 null [71], concluding that the protective action is afforded by the increase of AR
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