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Multiplex assay (cell death, lysosomal dynamics, mitochondrial membrane potentia) Cell death was detected using Propidium iodide (PI, Thermo Fisher Scientific) or SYTOX Green (SYTOX, Thermo Fisher Scientific) which is excluded from viable cells but exhibits red fluorescence following a loss of membrane integrity and Hoechst 33342 (Hoechst, Thermo Fisher Scientific) which stains chromatin blue in all cells to count the total number of cells

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The aforementioned results showed that BPC157 reached its peak rapidly in beagle dogs and was rapidly eliminated after reaching its peak