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Cells were lysed in NP40 buffer and protein was quantified using Pierce BCA Protein Assay Kit (Sigma)

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J Endocrinol 219(3):183193 Wmeau J-L, Klein M, Sadoul J-L, Briet C, Vlayoudom-Cphise F-L (2018) Graves disease: introduction, epidemiology, endogenous and environmental pathogenic factors