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Key points: Formulation: Most commonly, cyanocobalamin or hydroxocobalamin in aqueous solution is used

This creates localized pressure, increases pain, and can cause the solution to track back along the needle path and leak out

Single-cell methylation profiling using nanopore sequencing or single-cell bisulfite sequencing represents a transformative advance in detecting sperm-specific epigenetic alterations associated with transgenerational inheritance (135)