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Oral B12 supplements rely on a complex absorption pathway in the gut involving a protein called intrinsic factor

Free Cu2+ is toxic (Fenton reactions, hydroxyl radicals), but the GHK-Cu complex is stable, diffusible and delivers copper in a targeted manner to copper-dependent metalloenzymes: lysyl oxidase (collagen/elastin crosslinking), Cu/Zn superoxide dismutase (SOD1, antioxidant defence), tyrosinase (controlled melanogenesis), dopamine-beta-hydroxylase

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Nonetheless, we will focus on reviewing experimental evidence, mostly from animal studies, to better understand the potential effects of hydrochlorothiazide and loop diuretics on key processes involved in glucose regulation