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In brief, plated cells were incubated for 30 min at 37 C, 5% CO 2 in the staining solution (HBSS containing 1 g ml 1 Hoechst 33342 (62247, Invitrogen, Thermo Fisher Scientific) and either 5 M CellROXTM, 1 M FerroOrange or 1 M BODIPY 581/591 C11)

No differences in 5-HT2A receptors in mid-life depression or LLD, lower 5HT2A receptors in mid-life depression in orbital-frontal cortex, and higher 5-HT2A receptors have been reported [109,110,111,112]

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Before your visit, make sure your doctor knows about all the medications and supplements you take