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The LC-MS analysis employed state-of-the-art equipment, including an UHPLC (1290 Infinity LC, Agilent Technologies) coupled with a quadrupole time-of-flight (AB Sciex TripleTOF 6600) at Shanghai Applied Protein Technology Co., Ltd.Utilizing both HILIC and RPLC separation techniques, chromatographic separation ensured robust performance in both ESI positive and negative modes

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In total, the degradation of one molecule of palmitic acid generates approximately 130 molecules of ATP