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Cells were lysed using Mammalian cell lysis reagent (Pioneer, Shanghai, China) at 48 h post-transfection, and the extracted proteins were divided into 3 groups including Input, IgG and IP groups, to which 20 L of Protein A+G magnetic beads (Beyotime, Shanghai, China) and 5 g of antibody (IgG or the corresponding primary antibody) were added respectively and bound on a vertical rotator at 4 C for 12-18 h

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