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However, if the concentration of a metabolite that is distal to the metabolic reaction is expressed as a ratio to a metabolite that is proximal to the metabolic reaction, that ratio may reveal a change in that pathway that could otherwise go undetected

ThermoFisher, USA), the cells were fixed for 15 min in 4% paraformaldehyde and permeabilized using 0.1% Triton X-100 (T8787