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Benzing WC, Mufson EJ, Jennes L, Armstrong DM (1990) Reduction of neurotensin immunoreactivity in the amygdala in Alzheimers disease

5 and 6, samples in SDS solubilizer (0.0625 M TrisHCl (pH 6.8), 10% glycerol, 0.01% bromophenol blue, 2% (wt/vol) SDS, +/- 2% 2-mercaptoethanol) were heated at 95 C for 5 min, electrophoresed through 8% or 10% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad, USA), and incubated with SARS-CoV-2 (2019-nCoV) S antibody rabbit PAb antigen affinity purified, mouse monoclonal anti-SARS-S2 conjugated to HRP, goat anti-human IgG-HRP, ACE2 recombinant rabbit monoclonal antibody, or purified LgBiT-substrate cocktail

The five metrics of machine learning were applied to evaluate the performing algorithm, including accuracy, sensitivity, specificity, positive predictive value, and negative predictive value, which were defined as follows: (49)

[DOI] [PubMed] [Google Scholar] 5.Cabello CM, Bair WB, III, Wondrak GT