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Measure At 3.0 mL reconstitution of a 5 mg vial, 1 unit on a U-100 syringe is roughly 16.67 mcg

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Neelakantan H, Vance V, Wetzel MD, Wang HY, McHardy SF, Finnerty CC, Hommel JD, Watowich SJ. Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high-fat diet-induced obesity in mice. Biochem Pharmacol. 2018 Jul;154:161-171. doi: 10.1016/j.bcp.2018.05.003. Epub 2018 May 5. PMID: 29738706; PMCID: PMC6015571

To understand the underlying molecular mechanisms, the expression levels of 3-AR mRNA in both white and brown adipose tissue were assessed using reverse transcription followed by PCR and Southern blotting