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Technical Bridging Studies Transitioning from an IV vial to an SC injection device usually involves a change of the formulation to one with a higher API concentration to account for the lower bioavailability and limited volume of SC delivery

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2 Materials and methods 2.1 Drugs and reagents Ketamine (Vetased R , Pasteur, Romania), Thiopental, Ethylenediaminetetraacetic acid (EDTA), thiobarbituric acid (TBA), 1,1,3,3-tetraethoxypropane (TEP), vanadium (III) chloride (VCL3), sulfanilamide, N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDD), sodium nitrite (NaNO2), 2,4-dinitrophenylhydrazine (DNPH), trichloroacetic acid (TCA), guanidine hydrochloride, 5,5-Dithiobis-2-nitrobenzoic acid (DNTB)