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The authors performed ChIP-Seq using expression constructs that yield AR DBD domains that are linked to functional domains that convey transcriptional activation or repression, both in benign and malignant prostate cells and organoids
Simultaneously, released Cu 2+ causes redox reaction with the intracellular overexpressed GSH, and subsequently produces Cu + for initiation of Fenton-like reactions, resulting in the production of OH and robust anticancer therapy
The DNA-bound PARPs, such as PARP1-3, can attach the mono-ADP-ribose (MAR) or poly-ADP-ribose (PAR) moieties directly to the DNA breaks
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