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We specified that the nucleophilic atoms reacting are the hydroxyl elements of the CAA addressed by the oxygen atom as the most negatively charged molecule toward the vitamin B family hydrogens, which introduced as the electrophilic atoms, the CAA answerable for the nucleophilic attack are Asn142 with 1.95 , Cys145 with 3.71 , for thiamine, pyridoxine, individually, and His164 for both biotin with 1.80 , and with pantothenic acid with 1.83

The result: increased mitochondrial DNA quantity, enhanced cellular respiration, and elevated activities of key enzymes including citrate synthase and cytochrome c oxidase

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