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2.12 Shotgun metagenomic sequencing Fecal DNA ( n = 3/group) was extracted using a FastPure Stool DNA Isolation Kit (Magnetic Beads, MJYH, China), and its quality was assessed for concentration, purity, and integrity prior to fragmentation (400 bp) using a Covaris M220

-carnitine-fed C57BL/6 J mouse model The handling of animals complied with the guidelines of the Institutional Animal Care and Use Committee of National Taiwan University (approval number: NTU107-EL-00170 and NTU107-EL-00084)

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