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Herein we examined the role of GLP-1RAs and the novel dual GIP/GLP-1RA TZP as an add-on to basal insulin therapy compared to basal insulin alone in improving glucose control

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After treatment, cells were lysed with lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% Triton, 1 PIC), and treated with recombinant His8-USP7 (E-519-025, Bio-techne) at a final concentration of 20 nM for 6 hours at 37C