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After 24 h, mice were returned to the arena, in which one of the objects was placed in a new location (testing day, day 2)

It is commonly prescribed for individuals experiencing age-related declines in GH levels, which can affect metabolism, energy, muscle growth, and overall well-being

The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software

100 to 300 nm distance range from the Bassoon peak was considered as proximal vesicle pool and 400 to 800 nm as distal vesicle pool