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Our previous research demonstrated that U0126 can inhibit the ERK pathway by reversing the induced levels of pERK1/2 in PFOS-treated RTCs 3

Using 2mL gives you 2.5mg/mL concentration (20 IU = 0.5mg), while 2.5mL gives 2mg/mL (25 IU = 0.5mg)

This cleavage ultimately leads to a caspase-dependent cell death, which can be prevented by pan-caspase inhibitor Z-VAD-FMK (Khairalla et al., 2015)