( E H ) MDA-MB-231 cells transfected with siCTR or 2 different combination pools of 4 individual siRNA for FADS1 (siFADS1_pool1 and pool2) and FADS2 (siFADS2_pool1 and pool2) and assessed by qRT-PCR ( E ) were treated ON with 0.25 M RSL3 ( F ) or 2 h with 1 M RSL3 ( G , H ) and assayed for cell survival ( F ), confocal analysis ( G ), and cytofluorimetric analysis ( H ) to measure lipid peroxidation
Biochemical and Biophysical Research Communications , 219, 21-25
They accumulate in various tissues, e.g., in the thymus (Muller, 1977), uterus (Gouon-Evans, 2001), lungs (Mesnil et al, 2016), adipose tissue (Wu et al, 2011), mammary gland (Gouon-Evans et al, 2000) and gastrointestinal tract (Chu et al, 2014), whereas the peripheral blood contains only few eosinophils under homeostatic conditions
Simplified Purification Process The GST tag simplifies the purification process through its specific binding to glutathione