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Imaging data capture and analysis OB and LH sections processed for cFos in situ hybridisation were imaged with a Slide Scanner (Nanozoomer 2.0 HT, Hamamatsu, Japan)

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However, prolonged exposure above 86F can begin degrading the peptide

Ethical use of vertebrate animals All experiments using mice were performed in accordance with relevant guidelines and regulations specified in the Animal Welfare Act (AWA) (7 U.S.C