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Reusing equipment could compromise sterility and patient safety

At least in some cases, the same initiating mutation has been identified in both the melanocytic and vascular birthmark, suggesting that phakomatosis pigmentovascularis is a clonal process with two distinct lines of differentiation, perhaps arising due to a mutation in a multipotent stem cell

To remove bubbles, hold the syringe needle-up and tap the barrel firmly with your fingernail

Dried membranes (Nytran Supercharge Positively Charged Nylon Membrane (Fisher Scientific)) were blocked in 5% low-fat milk in PBS for 1h and incubated with primary antibody (anti-5mC, Cell Signalling (28692S), 1:750 dilution) in 1% bovine serum albumin (BSA) at 4C overnight