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Transcriptional regulation of renal cytoprotective genes by Nrf2 and its potential use as a therapeutic target to mitigate cisplatin-induced nephrotoxicity
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For an accurate assessment of GSH concentration, the absorbance was measured against a sample in which, instead of 108 l of the supernatant from reaction mixture I, 108 l of the mixture are added, collected previously in the same way, but without GSH (90 l of Tris-HCl buffer with NaN 3 and EDTA + 10 l of phosphate buffer + 4 l H 2 O 2 solution + 20 l trichloroacetic acid solution)
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