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A method that allows isolation and enrichment of the transfection-positive cells is an effective solution

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An aliquot (150 l) of the treated cells was inoculated into 150 ml of fresh Starkey-S 0 medium, added with different amounts of CuSO 4 with the final concentrations of 0 mM, 5 mM, 10 mM, and 20 mM or different amounts of ZnSO 4 with the final concentrations of 0 mM, 40 mM, 80 mM, and 160 mM, respectively, cultivated at 40C, and shaken at 150 rpm