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PBMCs were again resuspended in warm RPMI with 5% human AB serum, and 2mL of PBMCs was aliquoted and stored for future assays while the rest was distributed at 6 x 10 5 cells/well onto 24-well plates (Corning, NY, USA) coated with.001% poly-lysine (Sigma, St

In addition, the characterized LASSS treatment did not stimulate autolysis of HGF as monitored by SDS-PAGE for degradation of 60-kDa -chain and 90-kDa proform of recombinant HGF incubated with LASSS (at 1:8000) at 25 for 2 h (see supplementary Figure 1b), providing another important basis for the availability of LASSS-HGF

K., & Lu, R.-B

"The efficacy and safety of menatetrenone in the management of osteoporosis: a systematic review and meta-analysis of randomized controlled trials"