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(i) aerobes (require free O 2 for decomposition of organic matter), (ii) anaerobes (does not require O 2 for decomposition), and (iii) facultative (can perform decomposition of organic material both in oxic and anoxic conditions) These are the main microorganisms that are present in biological WWT processes, namely, trickling filters and activated sludge processes
succinogenes was amplified through PCR using pET28a- as as the template
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