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Lysates were then centrifuged at 500 g for 5 min to separate the cytoplasm from nuclei

Although the thyroid gland is not the organ that has the most significant overall amount of Se in the human body, it does have the highest concentration of Se among all endocrine organs and, in fact, among all tissues regarding the amount of Se that it contains per gram [9]

When pretreating the cells with N -ethylmaleimide (NEM, 20 mM), a trapping reagent of thiol species, for 0.5 h, the signals from the cytoplasmic regions are decreased in both green and yellow channels (Figure 7DF)