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Flash frozen tissues were homogenized using a benchtop homogenizer (Precellys 24, Bertin Instruments) and bulk tissue RNA isolation was conducted using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturers instructions

Each batch is manufactured under controlled conditions and independently verified for purity and identity

The addition of B12 does not alter GS-441524 concentration, dosing calculation, or antiviral mechanism