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How can a laboratory select and use a pathway probe with enough confidence that observed biology is not simply an artifact of concentration drift, stability behavior, solvent mismatch, or assay interference

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The AT 1 and AT 2 receptor subtypes have been well characterized ( 4 subtype has only been partially sequenced ( Formation of Angiotensin Ligands Angiotensin peptides are derived from the precursor protein angiotensinogen via several enzymatic conversion pathways [Figure 1