At 2 h after plating, the plating medium was replaced with fresh culture medium consisting of DMEM supplemented with 1 mM sodium pyruvate, 100 units/ml penicillin, 100 g/ml streptomycin, 0.2 % (vol/vol) of bovine serum albumin fraction V (instead of FBS), 100 nM dexamethasone, and 1 nM insulin (SigmaAldrich #I9278)
Pediatr Res 52 : 900906 Bal MP, de Vries WB, van der Leij FR, van Oosterhout MF, Berger RM, Baan J, van der Wall EE, van Bel F, Steendijk P 2005 Neonatal glucocorticosteroid treatment causes systolic dysfunction and compensatory dilation in early life: studies in 4-week-old prepubertal rats
Pens should never be placed in checked luggage where they may freeze in the cargo hold
These findings underscore the multifaceted benefits of GLP-1 RAs therapy in patients with type 2 diabetes and AKD